Non-invasive detection and quantification of the parasitic ciliate Ichthyophthirius multifiliis by real-time PCR
نویسندگان
چکیده
منابع مشابه
Surface immobilization antigen of the parasitic ciliate Ichthyophthirius multifiliis elicits protective immunity in channel catfish (Ictalurus punctatus).
Channel catfish (Ictalurus punctatus) that survive infection with the parasitic ciliate Ichthyophthirius multifiliis acquire immunity to subsequent challenge and produce specific antibodies in serum that immobilize the parasite in vitro. Cellular surface protein antigens targeted by these antibodies are referred to as immobilization antigens (i-antigens). By using an immobilizing mouse monoclon...
متن کاملDetection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples wer...
متن کاملCytokine mRNA quantification by real-time PCR.
Real-time PCR represents a new methodology that accurately quantifies nucleic acids. This has been made possible by the use of fluorogenic probes, which are presented in two forms, namely hydrolysis probes (also called TaqMan probes) and hybridisation probes. We decided to apply this methodology to cytokine mRNA quantification and this led us to the development of a protocol that provides an ea...
متن کاملQuantification and Optimization of Candida albicans DNA in Blood Samples Using Real- Time PCR
Background: Candida albicans (C. albicans) is a major cause of candidaemia in people with impaired immunity. Blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. We established a real-time PCR assay for C. albicans detection in blood by LightCycler PCR and melting curve analysis. Methods: Five milliliter blood samples from...
متن کاملRapid Detection of COVID-19 by RT-LAMP PCR Technique and its Comparison with Real-Time RT-PCR Method
Rapid antigen and antibody, serological tests, and RT ‑ PCR-based molecular methods are widely used for the detection of microorganisms worldwide. Objectives: This study aimed by Isothermal nucleic acid amplification techniques to detect the covid-19. Methods: In this study, 200 samples of nasopharynx and oropharynx were collected from Jamaran Heart Hospital in Tehran. Covid -19 was exami...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2005
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao065251